
The Challenge of Isolating E. Coli and Salmonella from Sewers
Isolation and identification of E. coli and Salmonella spp required meterials. Sewage sample, EMB agar medium, MacConkey Agar medium, Nutrient-Agar medium, sterilized laminar air-flow chamber, incubator, hot-air oven, autoclave, weighing balance, refrigerator, sterile petri-plates, sterile test tubes for serial dilution, autoclaved distilled water, L-shaped bent-glass rod for spreading the inoculum, sterile non-absorbent cotton, sterile pipettes,…