Isolation and identification of E. coli and Salmonella spp

The Challenge of Isolating E. Coli and Salmonella from Sewers

Isolation and identification of E. coli and Salmonella spp required meterials. Sewage sample, EMB agar medium, MacConkey Agar medium, Nutrient-Agar medium, sterilized laminar air-flow chamber, incubator, hot-air oven, autoclave, weighing balance, refrigerator, sterile petri-plates, sterile test tubes for serial dilution, autoclaved distilled water, L-shaped bent-glass rod for spreading the inoculum, sterile non-absorbent cotton, sterile pipettes,…

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